Journal: iScience

Article Title: Mtb HLA-E-tetramer-sorted CD8 + T cells have a diverse TCR repertoire

doi: 10.1016/j.isci.2024.109233

Figure Lengend Snippet:

Article Snippet: mouse anti-human CD4 clone OKT4 BV650 , BD Bioscience , Cat. no: 750989; RRID: AB_2875058.

Techniques: Cell Isolation, Recombinant, Lysis, Transfection, Purification, Staining, Produced, TaqMan SNP Genotyping Assay, Plasmid Preparation, Software, Modification

Journal: Frontiers in Microbiology

Article Title: A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia

doi: 10.3389/fmicb.2010.00112

Figure Lengend Snippet: Analysis of host cell trafficking pathways during treatment with FR179254 . HEp-2 cells were treated as indicated, and trafficking pathways were analyzed as described in Section (A) Lipid droplet (LD) visualization with the neutral lipid stain Bodipy 493. (B) Fluid-phase uptake as measured by fluorescent dextran. (C) Live cell visualization of multivesicular bodies/late endosomes using CD63-GFP expressing HEp-2 cells. (D) Analysis of late endosomes/lysosomes in LAMP1-YFP transfected cells. (E) Exocytic trafficking as measured by incorporation of fluorescent sphingomyelin (SM) in Golgi and chlamydiae. (F) Exocytic trafficking of glycoproteins as measured by localization of CD4 on the surface of transfected cells. (G,H) Analysis of retrograde trafficking using fluorescently tagged cholera toxin subunit B (CTxB) after pulse-chase labeling. Arrows within (C) and (E) indicate inclusions. Note the lack of significant staining in (C) (inset) and the presence of staining in (E) , which is contrast to a published study (Beatty, ).

Article Snippet: For CD4, non-permeabilized cells were incubated with primary Ab mouse anti-human CD4 (R&D Systems, Minneapolis, MN, USA) followed by secondary goat anti-mouse Alexa488 (Invitrogen).

Techniques: Staining, Expressing, Transfection, Pulse Chase, Labeling

Journal: Human reproduction (Oxford, England)

Article Title: A monoclonal antibody, HCL-2, raised against human luteal cells reacts with apolipoprotein-B and detects the uptake of low density lipoprotein by luteinizing granulosa cells.

doi: 10.1093/humrep/13.4.936

Figure Lengend Snippet: Figure 1. HCL-2 antigen expression in corpus luteum on day 7 detected by indirect immunofluorescence staining. (A) Haematoxylin and eosin staining. (B) Staining with HCL-2 mAb. (C) Staining with anti-apolipoprotein-B mAb (MAB012). (D) Negative control stained with anti-TNP mAb. HCL-2 antigen and apolipoprotein-B were expressed on the granular-shaped structure near the nuclei in the cytoplasm of both large (LL) and small (SL) luteal cells. Both proteins were also detected along the cell membrane, showing the similar expression profiles. Original magnification 3120. Bar 5 100 µm.

Article Snippet: The immunoglobulin isotype was determined using an isotyping kit for mouse monoclonal antibodies (Serotec Ltd, Oxford, UK).

Techniques: Expressing, Staining, Negative Control, Membrane

Journal: Human reproduction (Oxford, England)

Article Title: A monoclonal antibody, HCL-2, raised against human luteal cells reacts with apolipoprotein-B and detects the uptake of low density lipoprotein by luteinizing granulosa cells.

doi: 10.1093/humrep/13.4.936

Figure Lengend Snippet: Figure 5. Detection of HCL-2 antigen on human luteinizing granulosa cells cultured in a medium with or without low density lipoprotein (LDL) by indirect immunofluorescence staining. (A–D) Culture with LDL. (E–H) Culture without LDL. (A, C, E and G) Phase-contrast pictures. (B and F) HCL-2 antigen. (D and H) Negative controls (anti-TNP mAb). HCL-2 antigen was clearly detected in the cytoplasm of human granulosa cells (arrows) cultured in the medium containing LDL for 3 days (B), whereas it was hardly detected in those cultured without LDL (F). Original magnification 3240. Bar 5 50 µm.

Article Snippet: The immunoglobulin isotype was determined using an isotyping kit for mouse monoclonal antibodies (Serotec Ltd, Oxford, UK).

Techniques: Cell Culture, Staining